# 12. DETERMINATION OF AMMONIA CONTENT IN GIVEN WATER SAMPLE

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## APPARATUS REQUIRED:

1. Measuring cylinder
2. Pipette
3. Conical flask
4. Spectrophotometer
5. Aluminum foil

## THEORY:

Ammonia is naturally present in surface water, ground water and domestic sewage. Ammonia is largely produced by the deamination of organic nitrogen containing compounds and hydrolysis of curer.

Nessler’s method:

The method is based on the reaction between NH3 and HgI42 tetra-idomercury (TI) dnion in alkaline solution.

NH3+2HgI42+3OH—–>NH2Hg2IO+7I+2H2o

## PROCEDURE:

Firstly 25ml of water sample was taken. 1 ml of Nessler’s reagent was taken and 1 ml of Rochelle’s salt was added on it. Similar procedure was followed for the standard solution.

## CALCULATION:

(x1,y1)= (0.3,0.75)

(x2,y2)= (0.7,1.75)

now,

y-y1=(y2-y1/x2-x1) (x-x1)

or,(y-0.75)= 1/0.4 (x-0.3)

or, 0.4y-0.3=x-0.3

or, y-0.75 = 2.5(x-0.3)

or, y=2.5x=0.75+0.75

=2.5x

Comparing with y=mx + c

m= 2.5

c= 0

The concentration of ammonia can be determined by

y= mx + c

y= absorbance of water

c= constant

x= concn of ammonia

m= slop

Now,

y= mx + c

1.236=2.5x+c

1.236= 2.5x

x= 1.236/2.5

x= 0.4444 mg/c

The absorbance (y) was found 0.645 in Saraswati water tap,

y=mx+c

Or, 0.645=2.5x+0

Or, 2.5x=0.645

x=0258 mg/c

## CONCLUSION:

The concentration of ammonia on botanical ponds and Saraswati sadan tap water might be suitable for fish and drinking respectively because they are in low concentration.

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