Microbiology syllabus

Syllabus fromBachelorNote

These syllabus are the updated syllabus and taken from the tribhuwan university Nepal. The syllabus maynot be same to other universities.

Tribhuvan University Institute of Science & Technology

Four Year B. Sc. (microbiology) Curriculum

Course Title : General Microbiology
Course No: MB 101 (Major
Nature of the Course: Theory
Year: I year
Full Marks: 100
Pass Marks: 35

Course Objectives

Course content

History and development of microbiology

Development of microbiology with reference to modern era, important discoveries, theories of spontaneous generation and germ theory of disease (Louis Pasteur, Robert Koch)

Classification and nomenclature of microorganisms

Classification schemes of living organism, differential characteristics of prokaryotic and eukaryotic microorganism. General principle of nomenclature, three and five kingdom concepts, basic understanding of classification of bacteria, viruses, fungi and protozoa, classification, nomenclature and characterization of bacteria according to Bergey's Manual of Systematic Bacteriology

Scope and applications of microbiology

Harmful and beneficial microorganism; applied microbiology (medical, public health, agricultural, food, microbial biotechnology, environmental, industrial, pharmaceutical microbiology)

Morphology of bacteria

Structure of bacteria, morphology and fine structure of cell organelles, differences between gram negative and gram positive bacteria

Growth and physiology of bacteria

Nutritional types of bacteria (photolithotrophic, chemolithotrophic, photoorganotrophic, chemoorganotrophic), entry of nutrients, passive and active transport, bacterial growth, growth curve, factors affecting growth

Isolation, enumeration and culture of bacteria

Types of bacteriological culture medium, techniques for isolation and enumeration of bacteria (streak plate technique, pour plate technique, spread plate technique, membrane filtration, most probable number method, direct microscopic count), biochemical tests of bacteria, methods of culture of aerobic and anaerobic bacteria, culture preservation methods


Introduction to metabolism, regulation and energy involvement in glycolysis (Embden Mayerhoff Paranas pathway), glycogenesis, glycogenolysis, TCA cycle, pentose phosphate pathway, Entner Doudoroff’s pathway, phosphoketolase pathway, fermentative pathways and electron transport system

Microbial genetics

Structure and function of prokaryotic DNA, genetic code, plasmids, concepts of bacterial genetics and role of RNA & DNA; bacterial recombination (transformation, conjugation and transduction), types and importance of mutation

Microscopy and different techniques of handling of microorganisms

Microscopes (light, stereo, dark field, phase contrast, electron, fluorescence), types of staining and nature of dyes/stains, different types of staining methods for microorganisms, aseptic techniques in microbiology

Techniques in control of microorganisms

Principles, procedures and applications of disinfection and sterilization-temperature, irradiation, ultrasonication, filtration, chemicals, antibiotics and chemotherapeutic agents

Introduction to virology

General structure (size, symmetry and shape) of virus, classification schemes, viral genetics, detection, enumeration (plaque assay, haemagglutination test, quantitative PCR, neutralization test), cultivation of viruses

Introduction to parasitology

Origin, morphology and classification of parasites (blood, tissue, intestinal parasites, soil transmitted helminths and nematodes), techniques of detection, enumeration and identification of protozoan and helminthic parasites

Introduction to mycology

Origin, morphology and medical classification of fungi. Techniques of isolation, morphological identification, culture and enumeration of yeast and mold